Nitrate reductase activity in chicory roots following excision

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Nitrate reductase activity in chicory roots following excision

In young chicory plantlets [Cichorium intybus L. Witloof cv. Flash), nitrate assimilation takes place mainly in the roots. Nitrate reductase activity (NRA) was measured in roots deprived of shoot control by excision and transferred into a sucrose-containing medium. Such a treatment resulted in a drop of about 60% of NRA within 3 h. The level of NR protein decreased after 12 h and the level of N...

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Analysis of nitrate reductase mRNA expression and nitrate reductase activity in response to nitrogen supply

Nitrate is one of the major sources of nitrogen for the growth of plants. It is taken up by plant roots and transported to the leaves where it is reduced to nitrite in the. The main objective of this research was to investigate stimulatory effects of sodium nitrate, potassium nitrate, ammonia and urea on the production/generation of the nitrate reductase mRNA in Triticum aestivum plants. The pl...

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Intercellular localization of nitrate reductase in roots.

Experiments were conducted with segments of corn roots to investigate whether nitrate reductase (NR) is compartmentalized in particular groups of cells that collectively form the root symplastic pathway. A microsurgical technique was used to separate cells of the epidermis, of the cortex, and of the stele. The presence of NR was determined using in vitro and enzyme-linked immunosorbent assays. ...

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Inheritance of Nitrate Reductase Activity

-Since the F1 hybrid (B14 X Oh43) had been shown to have a higher (heterotic) level of nitrate reductase activity than either inbred parent (B14 or Oh43), studies were undertaken to determine the mode of inheritance. Standard methods for determining MNendelian inheritance were used to study segregation for level of nitrate reductase activity of individual plants. The genetic material used was t...

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Synthesis and turnover of nitrate reductase in corn roots.

THE INDUCTION AND REINDUCTION OF NITRATE REDUCTASE IN ROOT TIP OR MATURE ROOT SECTIONS SHOW ESSENTIALLY A SIMILAR PATTERN: a lag, a period of rapid increase in enzyme activity and finally a period of relatively minor change. Both inductions are sensitive to 6-methylpurine and cycloheximide. Kinetic studies with 6-methylpurine suggest that the half-life of the messenger RNA for nitrate reductase...

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ژورنال

عنوان ژورنال: Journal of Experimental Botany

سال: 1997

ISSN: 0022-0957,1460-2431

DOI: 10.1093/jxb/48.1.59